Mouse Brain Lymphatic Vessels

This study provides evidence of lymphatic vessels in the mouse brain, specifically in the cortex, thalamus, and hippocampus. Using confocal microscopy, Western blotting, and real-time PCR, lymphatic vessels were identified by the expression of Lyve1, Prox1, and VEGFC, alongside endothelial markers CD31 and CD34. Paraffin-embedded brain slices from wild-type Balb/C mice were stained with antibodies against Lyve1, CD31, and CD34, revealing lymphatic vessels through 2D and 3D imaging. A coiled 3D structure of lymphatic vessels was observed in the hippocampus, indicating a complex drainage network. Western blot and real-time PCR analyses confirmed the presence of lymphatic and endothelial markers at the protein and mRNA levels. These findings demonstrate a lymphatic system extending from the meninges into deeper brain regions, offering insights into immune and waste clearance pathways in the central nervous system and potential therapeutic targets for neurological disorders.

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Publication Details

Journal
ACS Chemical Neuroscience
Published
2025-11-20
DOI
https://doi.org/10.1021/acschemneuro.5c00533
Citations
1
Primary Topic
Cerebrospinal fluid and hydrocephalus
Type
article
Field-Weighted Citation Impact
0.91
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Cerebrospinal fluid and hydrocephalus
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Mouse Brain Lymphatic Vessels

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article en
1 citations

Abstract

This study provides evidence of lymphatic vessels in the mouse brain, specifically in the cortex, thalamus, and hippocampus. Using confocal microscopy, Western blotting, and real-time PCR, lymphatic vessels were identified by the expression of Lyve1, Prox1, and VEGFC, alongside endothelial markers CD31 and CD34. Paraffin-embedded brain slices from wild-type Balb/C mice were stained with antibodies against Lyve1, CD31, and CD34, revealing lymphatic vessels through 2D and 3D imaging. A coiled 3D structure of lymphatic vessels was observed in the hippocampus, indicating a complex drainage network. Western blot and real-time PCR analyses confirmed the presence of lymphatic and endothelial markers at the protein and mRNA levels. These findings demonstrate a lymphatic system extending from the meninges into deeper brain regions, offering insights into immune and waste clearance pathways in the central nervous system and potential therapeutic targets for neurological disorders.

ACS Chemical NeuroscienceVol. 16(23)
Tabriz University of Medical Sciences (IR), Shiraz University of Medical Sciences (IR), Royan Institute (IR), Gulf Research Center (CH), Cell Technology (China) (HK), Institute for Advanced Medical Research (US), Prosthetic Design + Research (United States) (US), Stem Cell Technology Research Center (IR), West Kazakhstan Marat Ospanov State Medical University (KZ), Stem Cell Technology (Taiwan) (TW), Chinese People 's Liberation Army No. 85 Hospital (CN), Bushehr University of Medical Sciences (IR), Persian Gulf University (IR)
Openalex Percentile: Top 20%
Cerebrospinal fluid and hydrocephalus
0.91
DOISourceOpenAlex
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