Rapid and sensitive detection of Theileria equi using a novel integrated RPACRISPR/Cas13a lateral flow assay
The developed assay provides a rapid, precise, and equipment-free diagnostic platform suitable for both field and clinical environments. Although the current protocol relies on DNA extraction, future optimization will aim to enable direct detection from unprocessed blood samples, thereby further simplifying point-of-care diagnostics for equine piroplasmosis.
Authors
- Amjed Alsultan (ORCID: https://orcid.org/0000-0002-6471-4825)
- Hanène Belkahia
- Salah Mahdi Karim
- Mohammed Al-Saadi (ORCID: https://orcid.org/0000-0003-2751-862X)
- Mourad Ben Saïd (ORCID: https://orcid.org/0000-0001-5567-4721)
- Dhama Al-sallami (ORCID: https://orcid.org/0000-0002-6337-4590)
Institutions
- University of Al-Qadisiyah (IQ)
- Manouba University (TN)
Publication Details
- Journal
- Journal of Equine Veterinary Science
- Published
- 2025-11-11
- DOI
- https://doi.org/10.1016/j.jevs.2025.105732
- Citations
- 1
- Primary Topic
- Vector-borne infectious diseases
- Type
- article
- Field-Weighted Citation Impact
- 2.05