Novel floxed cannabinoid receptor 2 mouse line combines knockout capability with dual fluorescent reporters

This novel microglia-specific, inducible CB₂ knockout model is the first to combine a floxed CB₂ allele with reporter genes, an essential advancement given the lack of reliable CB₂ antibodies. The findings demonstrate the model's specificity and effectiveness, while highlighting important considerations regarding Cre-mediated effects and recombination specificity. Furthermore, the floxed mouse can be crossed with any Cre line to study CB₂ expression and function in various tissues. This model provides a powerful platform for advancing understanding of CB₂ roles in microglia and supports future exploration of CB₂-targeted therapeutic strategies.