Embryo vitrification disturbs the pre-implantation DNA demethylation process and affects hepatic functions to threaten long-term metabolic health in mice
Overall, our study uncovers the negative impact of vitrification on DNA methylation remodeling during early embryo development, which may partially account for the elevated susceptibility to metabolic diseases in offspring. Our findings also suggest that the Tet2 gene is a potential therapeutic target for improving the developmental competence of vitrified-thawed embryos.
Authors
- Yifeng Lin (ORCID: https://orcid.org/0000-0003-4411-8388)
- Xilin Shen (ORCID: https://orcid.org/0000-0001-8746-5801)
- Yanyun Ying (ORCID: https://orcid.org/0000-0001-5457-5318)
- Hongqing Liang (ORCID: https://orcid.org/0000-0002-5494-2197)
- Weimin Ci (ORCID: https://orcid.org/0000-0002-4111-5360)
- Jin Hao (ORCID: https://orcid.org/0000-0002-6685-2017)
- Feixia Wang
- Junyan Zheng
- Yifeng Liu (ORCID: https://orcid.org/0000-0003-4029-5328)
- Yazeed Allan
- Jiani Jin
- Ying Yue (ORCID: https://orcid.org/0000-0002-7999-0363)
- Yang Yang (ORCID: https://orcid.org/0000-0002-2630-6062)
- Chuan Chen (ORCID: https://orcid.org/0000-0002-5742-5099)
- Dan Zhang (ORCID: https://orcid.org/0000-0002-3513-8400)
- Sun Qing-yuan
- Yuli Qian
- Juan Liu (ORCID: https://orcid.org/0009-0001-4772-0326)
- Jianpeng Chen
Institutions
- Harvard University (US)
- Beijing Institute of Genomics (CN)
- Chinese PLA General Hospital (CN)
- Ministry of Education (TH)
- First Affiliated Hospital Zhejiang University (CN)
Publication Details
- Journal
- Journal of Advanced Research
- Published
- 2025-11-01
- DOI
- https://doi.org/10.1016/j.jare.2025.11.032
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00