Preparation and in vitro property evaluation of NO-HA@ICG self-assembled nanoparticles for gas therapy synergized with phototherapy

The synergistic effect of gas therapy and phototherapy is an innovative therapy in the field of cancer treatment with a unique therapeutic mechanism and application characteristics. NO improves the tumor blood vessels and facilitates the drugs to reach the cancer site, and the heat and ROS generated by phototherapy promote the release of NO and synergistically induce cancer cell Apoptosis. In this study, a self-assembled nanoparticle constructed by NO donor and HA was prepared by microfluidic equipment, and ICG was encapsulated in the nanoparticle and evaluated for its phototherapeutic effect and NO release performance. And the results showed that NO-HA@ICG NPs + laser group produced stronger ROS and NO than Free ICG and NO-HA@ICG NPs groups, which had a synergistic effect. The nanoparticle NO is expected to improve the tumor vascularity to facilitate the drug to reach the cancer site, and not only did the heat and ROS released by phototherapy promote the release of NO, but also synergistically induced the apoptosis of cancer cells with NO.