In Vivo SELEX: A Strategy for Identifying Ovarian Cancer-Specific Aptamers
Ovarian cancer is a significant and life-threatening malignancy among women worldwide. Selective tumor targeting by molecular agents is essential for the development of effective therapeutics and diagnostics. Recognizing the disparities between in vivo and in vitro environments, we have implemented an in vivo SELEX strategy to identify DNA aptamers capable of specifically localizing to ovarian tumors in situ. Aptamer L1 has emerged as a promising candidate with a high binding affinity to Insulin-like Growth Factor Binding Protein 3 (IGFBP3), a tumor-promoting protein overexpressed in ovarian cancer. Notably, aptamer L1 effectively downregulates IGFBP3 expression and suppresses ovarian cancer cell proliferation. The unique molecular characteristics of aptamer L1 underscore its potential as a theranostic agent for ovarian cancer.
Authors
- Fen-Sheng Qiu (ORCID: https://orcid.org/0000-0003-0499-8093)
- Weihong Tan (ORCID: https://orcid.org/0000-0002-8066-1524)
- Yuanming Shen (ORCID: https://orcid.org/0000-0002-0474-3472)
- Tao Zhu (ORCID: https://orcid.org/0000-0003-0830-2735)
- Xiaona Fang (ORCID: https://orcid.org/0000-0003-2545-6509)
- Hui Meng (ORCID: https://orcid.org/0000-0003-4061-2100)
- Zhaofeng Luo (ORCID: https://orcid.org/0000-0002-2740-8204)
- Guomiao Liao
- Ruibin Jiang (ORCID: https://orcid.org/0000-0001-6977-3421)
- Siru Zhang
- chanyuan li (ORCID: https://orcid.org/0000-0003-1485-670X)
- Zheng Zhang (ORCID: https://orcid.org/0000-0002-4800-1059)
- Longjie Li (ORCID: https://orcid.org/0000-0001-9419-9636)
- Weichang Chen
- Huarong Tang (ORCID: https://orcid.org/0000-0002-1034-3010)
Institutions
- Gynecologic Oncology Group (US)
- Sun Yat-sen University (CN)
- Hunan University (CN)
- Anhui Medical University (CN)
- Chinese Academy of Sciences (CN)
- Harbin Institute of Technology (CN)
- Wenzhou Medical University (CN)
- Zhejiang Cancer Hospital (CN)
- Theranostics (New Zealand) (NZ)
- Women's Hospital, School of Medicine, Zhejiang University (CN)
- Sun Yat-sen University Cancer Center (CN)
- Lifan (China) (CN)
- State Key Laboratory of Chemobiosensing and Chemometrics (CN)
- State Key Laboratory of Oncology in South China
- Zhejiang University (CN)
Publication Details
- Journal
- ACS Applied Materials & Interfaces
- Published
- 2025-11-11
- DOI
- https://doi.org/10.1021/acsami.5c17714
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00