Immunological Research of Interstitial Photodynamic Therapy Using Talaporfin Sodium in a Rat Intracerebral Glioma Model

The interstitial PDT (i-PDT) is a new method that is expected to malignant gliomas arising deep within the brain, which are difficult to remove. We performed the preclinical study using a rat intracerebral glioma model. that is expected to C6 glioma cells were implanted into the basal ganglia of rats, and 2-3 weeks later, talaporfin sodium (TPS) was administered intraperitoneally. After that, fine plastic optical fiber was punctured into the tumor tissue, and an appropriate dose laser light was irradiated into the tumor. The brain was removed 24-72 hours after the i-PDT and analyzed pathologically. Histological analysis showed that tumor necrosis and apoptosis was induced in the areas. Immunostaining showed that T cells or B cells don't stain, but macrophages and NK cells were stained within the tumor. An experimental system of i-PDT using TPS was established using malignant glioma cells transplanted into the rat brain.