Photodynamic Strategies for Combating Bacterial Biofilm-Associated Infections

Persistent and recurrent bacterial pharyngotonsillitis is a significant challenge, often linked to biofilm formation in tonsillar crypts. These biofilms contribute to antibiotic resistance, complicating treatment. Photodynamic Therapy (PDT) has shown promise in enhancing antibiotic effectiveness against planktonic bacteria, but its impact on biofilms requires further exploration. This study investigates the effects of PDT using curcumin (CUR) as a photosensitizer on Staphylococcus aureus biofilms. The focus is on CUR’s ability to penetrate biofilm layers and the subsequent changes in biofilm reformation. CUR penetration was found to vary with biofilm formation time and complexity, significantly reducing biofilm recovery after PDT. PDT altered the biofilm’s structure and metabolism, improving CUR uptake. PDT demonstrates the ability to disrupt biofilm integrity and hinder bacterial regrowth, offering potential as an adjunctive therapy for biofilm-associated infections.