Combination of photodynamic and sonodynamic therapy combating massive melanoma in pigs

Photodynamic (PDT), sonodynamic (SDT) and sonophotodynamic (SPDT) therapy are alternatives for conventional treatments against cancer. They are Reactive Oxygen Species (ROS) based treatments which the reactive species are able to induce to kill cells and tumor necrosis. Singlet pigs with massive skin melanoma were used. Photogem (2 mg/kg) intravenous was injected 6 hours before treatment. For the treatment, Sonidel SP300 with an effective radiation area (ERA) of 5.0 cm 2 was coupled to the surface of the tumor. A total dose of 100J/cm 2 was delivered by LED (630 nm). Treatments were conducted in two equal sections. A significant reduction of treated and non-treated tumor size was observed, while clinical signs showed increase in the health of the animal. A notable increase in weight was observed. The synergy between PDT and SDT, can be a good approach for melanoma control.