Sublingual immunization with E2-CD154 protein and the STING agonist c-di-AMP confers protection against classical swine fever virus in pigs
These findings provide evidence, in a large mammalian host such as the pig, that c-di-AMP functions as an adjuvant for a recombinant E2-CD154 protein delivered sublingually, enhancing immune responses consistent with protection against viral replication. Together, these results offer insights into the development of non-replicating, DIVA-compatible platforms against CSFV and support the rational design of next-generation subunit vaccines targeting viral pathogens relevant to both veterinary and human medicine.
Authors
- Llilianne Ganges (ORCID: https://orcid.org/0000-0002-8644-3560)
- Carlos A. Duarte (ORCID: https://orcid.org/0000-0001-5308-9676)
- María Pilar Rodríguez-Moltó (ORCID: https://orcid.org/0000-0002-8388-407X)
- Mario Pablo Estrada (ORCID: https://orcid.org/0000-0002-9776-719X)
- Alina Rodríguez-Mallón (ORCID: https://orcid.org/0000-0002-6950-6793)
- Yusmel Sordo-Puga (ORCID: https://orcid.org/0000-0002-0866-195X)
- Danny Pérez-Pérez (ORCID: https://orcid.org/0000-0002-8793-6826)
- Sara Puente-Marín (ORCID: https://orcid.org/0000-0002-8735-2855)
- Talía Sardina-González
- Liani Coronado (ORCID: https://orcid.org/0000-0003-4278-0589)
- Adriana Muñoz-Aguilera (ORCID: https://orcid.org/0009-0008-7161-4241)
- Cristina Riquelme
- Saray Heredia
Institutions
- Centre de Recerca en Sanitat Animal (ES)
- Centro de Ingeniería Genética y Biotecnología (CU)
- Instituto Colombiano Agropecuario (CO)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-11-20
- DOI
- https://doi.org/10.3389/fcimb.2025.1713724
- Primary Topic
- Animal Disease Management and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Centres de Recerca de Catalunya
- Generalitat de Catalunya
- Ministerio de Ciencia e Innovación