Construction of EpCAM overexpression and knockdown vectors and their implications in colorectal cancer research
This study establishes a robust dual-vector toolkit for reliable EpCAM manipulation, highlighting a novel exon-targeting strategy that mitigates the limitations of previous approaches. Our findings demonstrate that EpCAM is a master regulator of CRC aggressiveness, dictating proliferative and metastatic phenotypes in a cell context-dependent manner. The genetically defined models provide a validated platform for therapeutic screening and safety assessment, forming a foundational resource for advancing EpCAM-targeted therapies and diagnostic applications.
Authors
- Jingwei Duan (ORCID: https://orcid.org/0000-0001-6551-9127)
- Meng Ren (ORCID: https://orcid.org/0000-0002-5129-3347)
- Rina Su (ORCID: https://orcid.org/0000-0001-7975-3280)
- Lingjun Chen (ORCID: https://orcid.org/0000-0002-5792-1930)
- Bingping Wang (ORCID: https://orcid.org/0000-0002-2039-4356)
- Hulin Ma
- Shuang Zhang (ORCID: https://orcid.org/0000-0002-9492-6562)
- Hao Zhang (ORCID: https://orcid.org/0000-0001-5330-8218)
- Jie Zhou (ORCID: https://orcid.org/0000-0003-3196-8471)
- Yanwei Gao (ORCID: https://orcid.org/0009-0002-6706-8344)
Institutions
- Inner Mongolia Electric Power Survey & Design Institute (China) (CN)
- Inner Mongolia People's Hospital (CN)
- Inner Mongolia Medical University (CN)
Publication Details
- Journal
- Frontiers in Genome Editing
- Published
- 2025-11-20
- DOI
- https://doi.org/10.3389/fgeed.2025.1679698
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00