Determination of Phenolic Acids in <i>Salvia miltiorrhiza</i> by Vortex‐Assisted Matrix Solid‐Phase Dispersion Coupled With High‐Performance Liquid Chromatography

ABSTRACT A cost‐effective and straightforward vortex‐assisted matrix solid phase dispersion (VA‐MSPD) method was developed for extracting phenolic compounds from Salvia miltiorrhiza . UIO‐66‐NH 2 and 7 mL of 79% methanol aqueous solution containing 478 mM ionic liquid ([BMIM][BF4]) were selected as the dispersing sorbent and eluent, respectively. The optimal conditions were determined as follows: sample‐to‐dispersant mass ratio of 3.5:1, grinding time of 4 min, a vortex time of 2 min. Based on these results, response surface methodology (RSM) was further employed to investigate the effects of three key factors (sample‐to‐dispersant mass ratio, ionic liquid concentration, and methanol volume fraction) and their interactions on phenolic acids extraction efficiency. In addition, the combination of matrix solid‐phase dispersion and high‐performance liquid chromatography (HPLC) permitted the determination of four major natural polyphenolic compounds, including danshensu, rosmarinic acid, salvianolic acid B, and salvianolic acid A. The method demonstrated good linearity, showing limit of detection (LOD) of 0.13–0.28 µg·mL −1 and limit of quantitation (LOQ) of 0.42–0.92 µg·mL −1 . The developed vortex‐assisted matrix solid phase dispersion (VA‐MSPD) method showed higher extraction efficiency, greater convenience, and reduced time, making it an excellent choice for analyzing active components in traditional Chinese medicinal herbs.