Differential Profiles of <scp>IL</scp> ‐31‐ and <scp>IL</scp> ‐5/ <scp>IL</scp> ‐13‐Producing Cells Among Helper T Cells, With Subtle <scp>IL</scp> ‐31 Expression in Innate Lymphoid Cells

The authors declare no conflicts of interest. The data that support the findings of this study are available from the corresponding author upon reasonable request. Figure S1: The gating hierarchy for cytokine expression patterns in lymphocytes from cervical lymph nodes was shown. Figure S2: Focusing on CD3+CD4+ T cells purified from the lesional skin, IL-31-producing cells were identified. A number of IL-13 and IL-5-producing cells could also be detected; however, IL-31-producing cells produced little to no IL-13 or IL-5. ILCs exhibited the production of IL-13 and IL-5, but showed little IL-31 expression. Figure S3: The cytokine profiles in the lymphocytes purified from skin-draining lymph nodes in MC903-treated and control ethanol-treated mice were shown. CD3+CD4+ T cells revealed a few populations of IL-31-producing cells. Some IL-13 and IL-5-producing cells could also be detected. ILCs exhibited the production of IL-13 and IL-5, but showed minimal expression of IL-31. Data S1: The names of the clones, fluorophores, suppliers, and dilution ratios of the antibodies used in the flow cytometry analysis are listed. Data S2: Papers suggesting potential differences in transcription factors, differentiation pathways, and environmental factors between IL-31-producing CD4+ T cells and IL-5/IL-13-producing Th2 cells were presented. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.