A modified α-synuclein seed amplification assay in Lewy body dementia using Raman spectroscopy and machine learning analysis
This preliminary study demonstrates that combining Raman spectroscopy with machine learning can enable rapid, label-free detection of disease-specific changes. Despite the very limited sample size, these findings highlight the potential of this novel workflow and strongly warrant its validation in larger cohorts.
Authors
- Lucy Butler (ORCID: https://orcid.org/0000-0002-7220-9634)
- David J. Koss (ORCID: https://orcid.org/0000-0002-8034-9880)
- Karunakaran Kalesh (ORCID: https://orcid.org/0000-0002-9421-3992)
- Tiago F. Outeiro (ORCID: https://orcid.org/0000-0003-1679-1727)
- Suzan Elsheikh
- Claudio Angione (ORCID: https://orcid.org/0000-0002-3140-7909)
- Agathe Quesnel (ORCID: https://orcid.org/0000-0002-8365-7686)
- Ojodomo J. Achadu (ORCID: https://orcid.org/0000-0002-5542-3110)
- Ahmad A. Khundakar (ORCID: https://orcid.org/0000-0002-4835-5359)
- Alan Thomas (ORCID: https://orcid.org/0000-0002-6667-9533)
- Jon Marles‐Wright (ORCID: https://orcid.org/0000-0002-9156-3284)
- Meez Islam (ORCID: https://orcid.org/0000-0002-6858-6963)
- Panagiota S. Filippou (ORCID: https://orcid.org/0000-0003-3974-988X)
- Annalisa Occhipinti (ORCID: https://orcid.org/0000-0001-6075-1496)
- Nathan Coles
- Alaa Gouda
Institutions
- University of Dundee (GB)
- German Center for Neurodegenerative Diseases (DE)
- Centre de Recherches en Cancérologie de Toulouse (FR)
- NIHR Newcastle Biomedical Research Centre (GB)
- Newcastle University (GB)
- Teesside University (GB)
Publication Details
- Journal
- Journal of Neuroscience Methods
- Published
- 2025-11-15
- DOI
- https://doi.org/10.1016/j.jneumeth.2025.110617
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Teesside University
- Newcastle University