Impact of Cinnamon Ethanolic Extract on Sperm Cryopreservability and Fertility Potential in Buffalo Bulls

The current research aimed at exploring the action of Cinnamon zeylanicum Bark ethanolic extract on sperm freezability as well as fertility in buffalo bulls. Cinnamon zeylanicum Bark ethanolic extract preparation, assay of its antioxidant status by DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging technique , HPLC (High Performance Liquid Chromatography) analysis to assess the concentrations of its flavonoids and phenolics. Semen samples were harvested from five healthy buffalo bulls. Tris extender with zero cinnamon was kept as a control. The other extenders were Tris containing ethanol extract of cinnamon at concentrations 4, 8, 16 mg/dl as experimental extender. The semen specimens were supplementary and neat sperm cell concentration 60 × 106/mL was fulfilled. Diluted semen was filled into 0.25 ml polyvinyl straws and exposed to the freezing process. Diluted semen was evaluated for sperm parameters after refrigeration and freeze-thaw process .The total phenolic content of Cinnamon Ethanolic extract (8256.62 µg/g extract, Cinnamic acid). The total flavonoids content of Cinnamon extract was 334435.79 µg/g extract, Daidzein and Hesperetin). The DPPH of Cinnamon Extract (91.9%) with 1000µg/ml and decreased with decreasing concentration. HPLC chromatograms of cinnamon extract with peaks of Daidzein, Cinnamic acid and Hesperetin. Spermatozoal measures were ameliorated post cooling and thawing especially in TC1. Improvement of conception rate was obvious in TC1 with elevated total antioxidants and decreased Malondialdehyde (MDA). The best concentration of ethanol extract of cinnamon that preserved high quality of spermatozoa, ameliorated post cooling and post freeze-thawing semen quality, and achieved the highest conception rate of buffalo semen extended in Tris dilluent was TC1 containing 4 mg/dL.