From Sample to Sequencing: The Importance of Pre-Analytical Sample Treatment in NGS Analysis of Patients with Chronic Lymphocytic Leukemia
The study demonstrates that inadequate starting material for DNA isolation may mask low-frequency <i>TP53</i> mutations, resulting in false-negative results. Accurate detection requires ensuring sufficient CD19+ cell content, which is critical for reliable diagnostics and supports personalized treatment approaches in CLL.
Authors
- Nikolina Kolobarić (ORCID: https://orcid.org/0000-0002-5649-8317)
- Vlatka Periša (ORCID: https://orcid.org/0000-0003-4801-1270)
- Saška Marczi (ORCID: https://orcid.org/0000-0003-2110-3327)
- Mirjana Suver Stević (ORCID: https://orcid.org/0000-0002-5418-3178)
- Hrvoje Holik
- Marina Samardžija
Institutions
- Croatian Institute of Transfusion Medicine (HR)
- University of Slavonski Brod (HR)
- University of Osijek (HR)
Publication Details
- Journal
- Cancers
- Published
- 2025-11-15
- DOI
- https://doi.org/10.3390/cancers17223668
- Primary Topic
- Chronic Lymphocytic Leukemia Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00