Effect of Crude Alkaloid Extract of Cladophora glomerata (L.) kutz. OnGene Expression and Cell Death

The objective of this study was to determine the physiological effects of the crude alkaloidextract from Cladophora glomerata at the half inhibitory concentration (300, 100, and 1000)μg /ml on the growth rate in the gene expression of the heat shock proteins (Hsp90, Hsp70,Hsp60), as well as the cell death genes (Caspase-8, P53, and Bcl2) of the cancer cell linesHepatocellular carcinoma (HepG2), human cervical cancer (Hela), and mice embryo fibroblast(MEF). In addition to monitoring morphological changes using real-time polymerase chainreaction for gene analysis using Kapa syber green dye, and observing morphological changesusing Acridine Orange / Propidium Iodid dye that examined using a fluorescence opticalmicroscope. The results of this study showed that treating cancer cell lines with the crudealkaloid extract at half inhibitory concentration effectively suppresses the gene expression ofheat shock protein that encode genes and increases the expression of programmed cell deathgenes. We conclude that Cladophora glomerata extract plays a major role in inhibiting cancercells (HepG2 and Hela cell lines) through regulating heat shock protein genes (Hsp90, HSp70,HSp60) and apoptosis genes (P53, Bcl2, and Caspase- 8) which have a major role in cancer.