Comparison and Quantification of Moxifloxacin in Pharmaceutical Formulas Using Aqueous, Lactic Acid and Choline Chloride‐Lactic Acid Media

Abstract This work demonstrates a validated and well‐established analytical methodology for quantifying Moxifloxacin (MOXF), as it is a native fluorescent pharmaceutical. As the most prescribed antibiotic, MOXF is focused on in terms of quantification in a number of pharmaceutical formulas in the current study. Herein, quantification of MOXF is based on the direct fluorescence measurement and comparison in three different media: namely, an aqueous (pH = 8.3), Lactic acid (Lac), and Lactic acid‐Choline chloride (Lac‐ChCl). The intensity of the fluorescence emission for the MOXF is measurable at 505, 510, and 515 nm after exciting at 290, 300, and 300 nm with respect to aqueous, Lac, and Lac‐ChCl media. Under the aforementioned conditions, the methodology has been given validity as the MOXF can successfully be quantified accurately and precisely within the linear range of 0.1–1.5 mg L −1 using Lac‐ChCl with a detection limit and quantification limit of 0.088 and 0.098 µg L −1 , respectively. The MOXF in various samples has been quantified, such as in Moxday, Muxava, Moksine, and RT‐MOXF as well as blood serum. The use of Lac‐ChCl as the chosen medium of analysis of MOXF in pharmaceutical products and spiked samples can be straightforwardly implemented.