Light‐Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers
In this study, a systematic approach is presented for the rational design of synthetic allosteric DNA aptamers. This methodology enables precise control over the allosteric ON-OFF transition in fluorescent DNA aptamers, allowing for the engineering of aptamers with highly tunable fluorescent properties. When combined with toehold-mediated strand displacement, a series of allosteric aptamers is developed in which the target sequence functions as a specific allosteric modulator. Furthermore, these aptamers are applied in synthetic DNA computing and in the construction of responsive nanostructures that light up upon activation.
Authors
- Da Han (ORCID: https://orcid.org/0000-0002-0804-2964)
- Bryan Wei (ORCID: https://orcid.org/0000-0003-2515-2409)
- Zhaoli Gao (ORCID: https://orcid.org/0000-0003-3114-2207)
- Wenwen Zeng (ORCID: https://orcid.org/0000-0001-8544-3318)
- Huangchen Cui
- Tanxi Bai
- Xinmin Qian (ORCID: https://orcid.org/0000-0003-1317-9292)
- Jiayi Zhang (ORCID: https://orcid.org/0000-0002-9766-8597)
- Wen Wang (ORCID: https://orcid.org/0000-0001-6344-407X)
- Yuxin Wang
- Tianqing Zhang (ORCID: https://orcid.org/0000-0002-8783-4416)
Institutions
- BGI Group (China) (CN)
- Chinese University of Hong Kong (HK)
- Chinese Academy of Sciences (CN)
- Hangzhou Academy of Agricultural Sciences (CN)
- China Datang Corporation (China) (CN)
- Beijing Meteorological Bureau (CN)
- Center for Life Sciences (CN)
- BGI Research (CN)
- Tsinghua University (CN)
Publication Details
- Journal
- Advanced Materials
- Published
- 2025-11-17
- DOI
- https://doi.org/10.1002/adma.202514306
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00