A comparative analysis between real‐time polymerase chain reaction and next‐generation sequencing for the simultaneous detection of key fusions in acute myeloid leukaemia

The authors declare no conflict of interest related to this work. All data generated or analysed during this study are available within the manuscript and its Supporting Information S1. Figure S1. Plasmid map of the (A) BCR::ABL1p210p190p23, (B) PML::RARAbcr1, (C) PML::RARAbcr2, (D) PML::RARAbcr3, (E) RUNX1::RUNX1T1, (F) KMT2A::MLLT1, (G) KMT2A::MLLT3, (H) KMT2A::MLLT10 and (I) ABL1. Figure S2. Melting curve analysis of real-time polymerase chain reaction to assess amplification specificity of the products (A) BCR::ABL1p210, (B) BCR::ABL1p190, (C) ABL1, (D) PML::RARA, (E) CBFB::MYH11, (F) RUNX1::RUNX1T1, (G) KMT2A::MLLT1, (H) KMT2A::MLLT3 and (I) KMT2A::MLLT10. Table S1. Sequence of the gene fusions investigated in the study. Table S2. Primers and probes used for identifying gene fusions by real-time polymerase chain reaction. Table S3. Optimization of annealing temperature, primer and probe concentration and extension time for real-time polymerase chain reaction. Table S4. Assessment of primer efficiency in real-time polymerase chain reaction amplification. Table S5. Number of positive control plasmid copies per reaction and per microlitre for each target fusion gene. Table S6. Assessment of repeatability and reproducibility of real-time polymerase chain reaction. Table S7. Assessment of the limit of detection of real-time polymerase chain reaction. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.