Towards Electrophysiological and Histological Mapping of Upper Limb Nerves in Pigs Using Epineural Stimulation

Understanding the relationship between nerve anatomy and the functional outcomes of electrical stimulation is critical for optimizing neural interface design. In this study, we conducted acute experiments on four pigs in which epineural cuff electrodes with multiple contacts were placed around upper limb nerves. A subset of electrical stimulation configurations -- previously identified via computational study -- was applied, and the resulting evoked electromyographic (EMG) responses were recorded from target muscles. Muscle recruitment curves were extracted and analysed offline to quantify activation patterns. Following the electrophysiological experiments, the stimulated nerves were harvested and processed for histological analysis to visualize fascicular organization and distribution. This work presents preliminary results from the combined analysis of muscle activation profiles and fascicle anatomy in one animal. Our findings aim to inform the design of stimulation strategies by linking electrode configuration to selective muscle recruitment, ultimately contributing to more effective neuromodulation and neuroprosthetic applications.