A sensitive and specific assay to characterize plasma kallikrein activity in plasma from patients with hereditary angioedema
We developed a specific PKa assay that can detect low levels of PKa activity in plasma and can differentiate patients with HAE-C1INH from controls without HAE with high sensitivity and specificity. Using this assay, we demonstrated that sPKa activity is elevated during the intercritical period in patients with HAE-C1INH and in those with HAE-nC1INH compared with controls when measured after 6 h of cold incubation. This sensitive and specific PKa assay could be useful to characterize PKa activity in plasma samples from patients with HAE and could potentially serve as a future candidate biomarker for HAE-nC1INH.
Authors
- Nivetha Murugesan (ORCID: https://orcid.org/0009-0000-4386-5563)
- Arije Ghannam (ORCID: https://orcid.org/0000-0002-5045-0566)
- Danny M. Cohn
- Edward P. Feener (ORCID: https://orcid.org/0000-0003-1175-8469)
- Daniel K. Lee (ORCID: https://orcid.org/0000-0002-6623-6089)
- Marta Donà
- Sally L. Hampton (ORCID: https://orcid.org/0000-0002-4305-5461)
- Paul Audhya
- Adil Adatia (ORCID: https://orcid.org/0000-0002-7612-6529)
- Michael D. Smith
- Vincent Denis
Institutions
- University of Alberta (CA)
- Université de Montpellier (FR)
- Allergan (Ireland) (IE)
- CEA Grenoble (FR)
- KalVista Pharmaceuticals (United States) (US)
- Allergan (France) (FR)
- Services déconcentrés d'appui à la recherche Occitanie-Montpellier (FR)
- Amsterdam University Medical Centers (NL)
- University of Amsterdam (NL)
Publication Details
- Journal
- World Allergy Organization Journal
- Published
- 2025-11-01
- DOI
- https://doi.org/10.1016/j.waojou.2025.101135
- Primary Topic
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Type
- article
- Field-Weighted Citation Impact
- 0.00