Standardization and Development of Standard Operating Procedures (SOPs) of a Polyherbal Unani Formulation Qurṣ-i Bawāsīr

Qurṣ-i Bawāsīr (QB) is a highly potent compound Unani formulation as it has synergistic effect in the treatment of Bawāsīr (Piles). Its ingredients act as anti- inflammatory, analgesics, purgative and emollient. The present study aims at identification and authentication of the crude ingredients of QB, development of SOPs and HPTLC fingerprinting by modern analytical techniques to comprehend its quality. QB in its three different batches was evaluated for organoleptic parameters, microscopic studies, physicochemical parameters, phytochemical screening, TLC and HPTLC profile, aflatoxin, microbial load and heavy metal analysis. QB was successfully standardized with all the parameters such as total ash, acid insoluble ash, water and alcohol soluble matter and loss of weight on drying. Further, TLC and HPTLC fingerprinting profile was developed and detected under various detection systems, viz. UV 366nm, UV 254nm, exposure to iodine vapours and anisaldehyde sulphuric acid. The present study ensured authenticity of the Unani formulation and furnished referential facts for its identification and purity thereby substantiating a validated scientific data. Keywords: Qurṣ-i Bawāsīr, Unani formulation, Standardization, HPTLC