Modern approaches to the construction and use of recombinant viruses
The review describes certain viral vectors and considers various methods for constructing recombinant viruses with special attention paid to the homologous recombination and CRISPR/Cas9 system, and also describes the capabilities of using various cloning vectors (different plasmids, BAC etc.). The review also presents a comparative analysis of the effectiveness and safety of using various viral vectors, both for creating recombinant vaccines and for obtaining oncolytic viruses, as well as medicines for gene therapy.
Authors
- Н. Н. Власова (ORCID: https://orcid.org/0000-0001-8707-7710)
- Anton Yuzhakov (ORCID: https://orcid.org/0000-0002-0426-9678)
- Alexey Gulyukin (ORCID: https://orcid.org/0000-0003-2160-4770)
- Polina V. Prokhorova (ORCID: https://orcid.org/0009-0009-7278-5541)
Institutions
- All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin (RU)
- All-Russian Scientific Research Institute of Experimental Veterinary Sciences. Ya.R. Kovalenko (RU)
Publication Details
- Journal
- Problems of Virology
- Published
- 2025-08-30
- DOI
- https://doi.org/10.36233/0507-4088-323
- Primary Topic
- Virus-based gene therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00