Protocol for creating a gene dictionary for organelle genomes using the Gene Dictionary Tool

Here, we present a protocol for creating a gene dictionary for fungal mitochondrial genomes using the Gene Dictionary Tool. Through a Python Command Line Interface (CLI), the user identifies what annotations are missing in the inputted dictionary. Via two Jupyter Notebooks, the user builds a gene dictionary based on attributes retrieved from inputted GFF3 files. The final output, a .gdict file, is findable, accessible, interoperable, and reusable (FAIR). This protocol can be adapted to create a gene dictionary for other genomes.