Recommendations for Studying <i>In Situ</i> Extracellular Vesicles From Solid Tissue
Solid tissue-derived extracellular vesicles (ST-EVs) are extracellular vesicles (EVs) separated directly from solid tissues of both vertebrates and invertebrates. ST-EVs provide a physiologically relevant snapshot of tissue-specific molecular dynamics and can be enriched directly in situ, from tissues in their natural state, preserving the native characteristics of ST-EVs. However, their enrichment presents unique technical challenges compared to EVs derived from biofluids or cell culture media. The need for transparent reporting in ST-EV research is crucial to enhance the reproducibility, comparability, and reliability of research findings. The Solid Tissue Task Force, part of the Scientific Reproducibility Subcommittee of International Society for Extracellular Vesicles, aims to recommend reporting parameters and identify outstanding questions related to the pre-analytical and analytical handling of solid tissues, as well as ST-EV separation and characterization. These steps are essential for advancing the understanding of the biological roles of ST-EVs and their potential clinical applications.
Authors
- Laura J. Vella (ORCID: https://orcid.org/0000-0002-1869-6291)
- Tsuneya Ikezu (ORCID: https://orcid.org/0000-0002-3979-8596)
- Yiyao Huang (ORCID: https://orcid.org/0000-0003-1749-963X)
- Rossella Crescitelli (ORCID: https://orcid.org/0000-0002-1714-3169)
- Andrew F. Hill (ORCID: https://orcid.org/0000-0001-5581-2354)
- Lucia Paolini (ORCID: https://orcid.org/0000-0002-4410-5272)
- Efrat Levy (ORCID: https://orcid.org/0000-0001-6890-6763)
- Stephanie N. Hurwitz (ORCID: https://orcid.org/0000-0002-6975-9168)
- An Hendrix (ORCID: https://orcid.org/0000-0002-1883-8309)
- Berta Puig (ORCID: https://orcid.org/0000-0002-2255-8393)
Institutions
- Nathan Kline Institute for Psychiatric Research (US)
- Indiana University Health (US)
- Johns Hopkins University (US)
- Universität Hamburg (DE)
- The University of Melbourne (AU)
- Florey Institute of Neuroscience and Mental Health (AU)
- Johns Hopkins Medicine (US)
- Sahlgrenska University Hospital (SE)
- Jacksonville College (US)
- Ghent University (BE)
- Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (IT)
- Surgical Specialties (Canada) (CA)
- Nanfang Hospital (CN)
- University Medical Center Hamburg-Eppendorf (DE)
- Cancer Research Institute Ghent (BE)
- Mayo Clinic in Florida (US)
- Indiana University School of Medicine
- Indiana University – Purdue University Indianapolis (US)
- New York University (US)
- Southern Medical University (CN)
- Victoria University (AU)
- University of Brescia (IT)
- University of Gothenburg (SE)
Publication Details
- Journal
- Journal of Extracellular Vesicles
- Published
- 2025-11-01
- DOI
- https://doi.org/10.1002/jev2.70185
- Citations
- 2
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Ministero della Salute
- Vetenskapsrådet
- Università degli Studi di Brescia