Monitoring the ethanol extraction characteristics of Taegeuk ginseng functional components

This study investigated optimal extraction conditions by monitoring ethanol extraction conditions to maximize the functional component extraction of Taegeuk ginseng through response surface analysis. The R2 of soluble solid, total ginsenoside, and acidic polysaccharide model formula of Taegeuk ginseng were 0.8894-0.9354 (p<0.05). The soluble solid and acidic polysaccharide of Taegeuk ginseng, according to the extraction conditions, showed a high yield under low ethanol content, but ginsenosides were well extracted under conditions with high ethanol concentration. The optimum extraction conditions for soluble solids were 0.22% ethanol concentration, 68.96°C, and the maximum extraction yield was 57.81%. The maximum extraction yield of total ginsenoside was 17.24 mg/g when Taegeuk ginseng was extracted at 77.03°C in 66.41% ethanol concentration. The optimum extraction conditions for ginsenoside Rg1, Rb1, Rb2, and Rb3 were in the range of 68.00-78.69% ethanol concentration and 70.04-75.68°C, and at this time, the content of each ginsenoside was all high in the range of 2.10-5.45 mg/g. Furthermore, the maximum extraction yield of the acidic polysaccharide was 19.03 mg/g when extracted at 71.37°C in 0.38% ethanol concentration. The extraction yield of Taegeuk ginseng functional components can simultaneously increase the yield of ginsenoside and acidic polysaccharide when extracted with 30-50% ethanol.