Storage conditions differentially alter the human tooth enamel proteome

Although -80°C storage remains the gold standard for preserving organic material, storage in 70% EtOH at RT produced comparable proteomic results. This suggests that ethanol-based storage protocols may serve as a more practical alternative, easier to implement for sample collection, and help provide consistency in enamel research. Our findings underscore the importance of both storage methods and standardized protocols in enamel proteomics, as they help avoid bias in protein detection and facilitate comparisons of datasets between studies.