Ex vivo perfusion model of mouse liver and its application to analyze the effects of OCT1 deficiency
The method enables reproducible and reliable perfusion of mouse livers. We applied it to study the effects of Oct1 knockout on drug metabolism. Oct1 knockout affected the first-pass metabolism of codeine, including the formation of the metabolites morphine and morphine-3-glucuronide, as well as the first-pass metabolism of proguanil and the formation of cycloguanil. The model is applicable to any mouse strain, genetic background, and substrate of interest and is thus applicable to a wide variety of research questions.
Authors
- Marleen J. Meyer (ORCID: https://orcid.org/0000-0002-3639-9303)
- Giuliano Ciarimboli (ORCID: https://orcid.org/0000-0002-4365-3656)
- Mladen V. Tzvetkov (ORCID: https://orcid.org/0000-0003-1670-5534)
- Markus Grube
- Vincent Rönnpagel (ORCID: https://orcid.org/0009-0000-3249-8614)
- Felix Morof (ORCID: https://orcid.org/0009-0001-3701-6247)
Institutions
- Universitätsmedizin Greifswald (DE)
- University Hospital Münster (DE)
Publication Details
- Journal
- Frontiers in Pharmacology
- Published
- 2025-11-14
- DOI
- https://doi.org/10.3389/fphar.2025.1629421
- Primary Topic
- Pharmacogenetics and Drug Metabolism
- Type
- article
- Field-Weighted Citation Impact
- 0.00