SKA1 knockdown by lentivirus inhibits proliferation and tumorigenesis in human osteosarcoma: Electrochemical sensor disease diagnosis

Osteosarcoma is a highly aggressive primary malignant bone tumor, especially threatening adolescents and young adults. Early diagnosis is crucial for improving patient survival rates. Traditional diagnostic methods like imaging exams and tissue biopsies have limitations such as long turnaround times and potential discomfort. However, electrochemical sensors are emerging as a promising alternative. These sensors offer high sensitivity, quick response, and cost-effectiveness, showing great potential in early disease detection.This study explores the application of electrochemical sensors in detecting osteosarcoma markers. It combines the sensor's analytical power with the effects of SKA1 gene knockout on osteosarcoma cells. Blood markers from osteosarcoma patients and healthy controls were analyzed to evaluate the sensor's performance. The results showed that electrochemical sensors could accurately differentiate osteosarcoma patients from healthy individuals, with higher sensitivity than traditional ELISA methods. They also demonstrated excellent linearity and stability in detecting other biomarkers.Electrochemical sensors have made significant progress in medical testing, showing great potential in osteosarcoma diagnosis and broader biomarker analysis. They are a transformative tool for improving disease diagnosis and patient care. • SKA1 plays a critical role in maintaining stable kinetochore-microtubule attachments during cell mitosis. • depletion of SKA1 via shRNA knock-down suppressed the growth, colony formation, and migration abilities of OS cells. • SKA1 played important roles in OS tumorigenesis.