Adhesive and Wound Healing, Dual Active Hydrogel with Snail Mucus Proteins

Many gastropods secrete mucus, which is more viscous and adhesive than the common trail mucus. The primary biochemical distinction between the two types of mucus is the higher protein content of the adhesive mucus. Not enough is known about the function of each of these proteins. In the current study, two of such mucus proteins were isolated from the adhesive mucus of the land snail <i>Macrochlamys indica</i>. In an attempt to imitate the structure of the mucus, these proteins were mixed with commercial hyaluronic acid (HA). The resultant hydrogel was found to have adhesive properties. A cell viability assay revealed that each of the hydrogel components and their mixtures were biologically safe and compatible. The <i>in vitro</i> cell migration assay showed better wound closure in case of the mucus protein as compared to HA, which is already known for its wound healing properties. The hydrogel was used for incision wound healing in mice, followed by histological staining. The result showed faster healing when compared to that of commercial wound healing ointment. In conclusion, this study presents a wound repair material, formulated from snail protein and HA and useful as an adhesive wound dressing with healing effects.