Investigating the Anticancer Effects of Sulforaphane in an In Vitro Coculture Model of Prostate Cancer Cells with Engineered Heart Tissue
Sulforaphane (SFN) is a phytoderived compound abundant in cruciferous plants that possesses a broad spectrum of anticancer properties. We showed that SFN-induced caspase-mediated apoptosis in grade IV bone metastasis-derived androgen-insensitive PC-3 (IC<sub>50</sub> = 4.2 μM), and lymph node metastasis-derived androgen-sensitive LNCaP (IC<sub>50</sub> = 2.8 μM) prostate adenocarcinoma cells. SFN-mediated cardiotoxic side effects were tested in a preclinical in vitro model that enables the study simultaneously of the impact of drugs on cancer cell death and contractile properties of engineered heart tissues generated from human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM EHT). Thereby, SFN exposure induced PC-3 cell death without affecting the contractile force of hiPSC-CM EHT. Interestingly, the irregular beating pattern of hiPSC-CM EHT observed in the presence of PC-3 coculture was normalized compared to vehicle treatment. Overall, this in vitro coculture model of hiPSC-CM EHT and cancer cells could facilitate the study of cardiotoxic cancer drug side-effects.
Authors
- Thomas Eschenhagen (ORCID: https://orcid.org/0000-0003-1750-1170)
- Laura Rathjens
- Tina Rohlfing
- Friederike Cuello (ORCID: https://orcid.org/0000-0003-1612-1715)
- Gunhild von Amsberg (ORCID: https://orcid.org/0000-0002-5176-0507)
- Jane In den Birken
- Κωνσταντίνα Σταθοπούλου (ORCID: https://orcid.org/0000-0002-3746-3055)
- Alexandra Rhoden (ORCID: https://orcid.org/0000-0002-9143-5522)
- Sergey A. Dyshlovoy (ORCID: https://orcid.org/0000-0002-7155-9245)
- Hannah Münch
Institutions
- Universität Hamburg (DE)
- Far Eastern Federal University (RU)
- University Medical Center Hamburg-Eppendorf (DE)
- Martini-Klinik (DE)
- University Cancer Center Hamburg (DE)
- German Centre for Cardiovascular Research (DE)
- Advanced Materials and Technologies (Slovenia) (SI)
Publication Details
- Journal
- ACS Pharmacology & Translational Science
- Published
- 2025-11-14
- DOI
- https://doi.org/10.1021/acsptsci.5c00622
- Primary Topic
- Genomics, phytochemicals, and oxidative stress
- Type
- article
- Field-Weighted Citation Impact
- 0.00