Tardigrade Dsup Protein Protects Human Cells during Proton Radiotherapy

Principles of resistance to physicochemical stresses in extremophile organisms are unique for the development of new protective technologies. In this work, Dsup (Damage suppressor) protein, which belongs to one of the most radiation-resistant animals—tardigrade Ramazzottius varieornatus, was studied as a perspective radioprotector for proton therapy. Dsup, known for its effective DNA-protective properties, was produced in human cells HEK293 expressing Dsup gene. To study radio resistance, human cells were irradiated by high-energy protons (150 MeV) at Medical-Technical Complex (MTC) of Phasatron (JINR). Within the actual medical protocol of proton radiation therapy, Dsup was confirmed to be the potential protector for healthy cells and tissues.