Development and validation of PmMAD7 for efficient gene editing in Penaeus monodon
PmMAD7 constitutes an enhanced CRISPR editing tool specifically designed for P. monodon, exhibiting superior precision, expanded PAM compatibility, and enhanced editing efficacy relative to conventional Cas12a systems. These results lay the groundwork for the advancement of gene editing applications in crustaceans and contribute to sustainable genetic improvements in aquaculture.
Authors
- Song Jiang (ORCID: https://orcid.org/0000-0002-9831-9030)
- Jianhua Huang (ORCID: https://orcid.org/0000-0001-9367-5186)
- Yundong Li (ORCID: https://orcid.org/0000-0002-4254-8635)
- Qibin Yang (ORCID: https://orcid.org/0009-0004-2840-2332)
- Lishi Yang (ORCID: https://orcid.org/0000-0001-8348-0641)
- Sheng Huang (ORCID: https://orcid.org/0000-0002-2868-4331)
- Erchao Li (ORCID: https://orcid.org/0000-0001-8495-2241)
- Jianzhi Shi (ORCID: https://orcid.org/0009-0007-8551-6573)
- Ziyi Jiang (ORCID: https://orcid.org/0000-0002-8511-2364)
- Falin Zhou (ORCID: https://orcid.org/0000-0001-7864-8411)
- Y. W. Ding
Institutions
- University of Southern California (US)
- Hainan Agricultural School (CN)
- Sanya University (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Hainan 301 Hospital (CN)
- Chinese Academy of Fishery Sciences (CN)
- East China Normal University (CN)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2025-11-12
- DOI
- https://doi.org/10.1186/s12896-025-01060-7
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Basic and Applied Basic Research Foundation of Guangdong Province
- Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences
- South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences