Environmental <scp>DNA</scp> ‐based <scp>RPA</scp> ‐ <scp>CRISPR</scp> /Cas12a assay for on‐site detection of chironomid larvae in aquatic environments
Our eDNA-based RPA-CRISPR/Cas12a assay addresses the limitations of traditional biomonitoring by requiring only a small sampling volume, thereby minimizing habitat disturbance, while offering much higher sensitivity. This assay represents a valuable tool for on-site detection of invasive species and for monitoring environmental and public health threats (e.g., chironomids). © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Authors
- Bhavya Kachiprath (ORCID: https://orcid.org/0000-0002-8172-1007)
- In‐Cheol Yeo (ORCID: https://orcid.org/0000-0001-8562-7213)
- Chang‐Bum Jeong (ORCID: https://orcid.org/0000-0002-5930-555X)
- Ihn–Sil Kwak (ORCID: https://orcid.org/0000-0002-1010-3965)
- K. Kim
- Kyu‐Young Shim
Institutions
- Chonnam National University (KR)
- Incheon National University (KR)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-11-13
- DOI
- https://doi.org/10.1002/ps.70382
- Primary Topic
- Environmental DNA in Biodiversity Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Incheon National University