Antifungal Mechanism Effect of Artemisinin on Fusarium solani

<b>Background:</b><i>Fusarium solani</i> (<i>Fs</i>), a drug-resistant phytopathogenic fungus, is a major cause of severe infections in both plants and humans. <i>Artemisia annua</i> and its derivatives exhibit antimicrobial, antiviral and anticholesterolemic activities, yet their clinical use has been dominated by potent antimalarial and anticancer effects. Artemisinin (ART), a sesquiterpene lactone isolated from <i>A. annua</i>, is well recognized for its antimalarial efficacy but remains underexplored as an antifungal agent. <b>Methods:</b> Conidia of <i>Fs</i> were treated with increasing concentrations of ART (75-500 μM) for 0 and 24 h. Fungal viability was assessed using viability assays. Membrane permeability was examined using confocal laser scanning microscopy with propidium iodide (PI) staining. Protein carbonylation assays were performed to quantify oxidative damage induced by ART. <b>Results:</b> A 24 h, ART exposure reduced <i>Fs</i> viability in a dose-dependent manner, with an IC₅₀ of 147.5 μM. At 500 μM, ART achieved fungicidal activity with 99% growth inhibition. Confocal microscopy confirmed extensive membrane disruption in ART-treated conidia, while carbonylation assays demonstrated marked protein oxidation, supporting a mechanism involving free radical generation from the peroxide bridge of ART. ART exhibits potent antifungal activity against <i>Fs</i>, mediated by oxidative stress, membrane disruption and protein carbonylation. <b>Conclusions:</b> These findings highlight ART as a promising candidate for antifungal drug development against resistant <i>Fusarium</i> species.