Cellular osmoregulation enhances porcine embryo development by restoring zygotic genome activation via metabolic–epigenetic crosstalk
These findings identify that mitochondria in porcine preimplantation embryos act as osmotic stress sensors. Under conditions of extracellular organic osmolyte deficiency and elevated osmolarity, they drive metabolic reprogramming and nuclear epigenetic dysregulation, ultimately disrupting mitochondrial-nuclear communication, compromising ZGA, and inducing developmental arrest. These findings provide mechanistic insights for optimizing in vitro culture systems in reproductive technologies.
Authors
- Xiangyuan Zhao (ORCID: https://orcid.org/0000-0003-0994-078X)
- Xiaoyu Chu (ORCID: https://orcid.org/0000-0002-7096-6076)
- Baozeng Xu (ORCID: https://orcid.org/0000-0001-9741-3407)
- Jing Shao (ORCID: https://orcid.org/0009-0002-3820-4458)
- Yu Tang (ORCID: https://orcid.org/0000-0001-6720-0734)
- Lixiang Liu (ORCID: https://orcid.org/0000-0001-6977-3990)
- Yifeng Yang
- Bingfeng Fan (ORCID: https://orcid.org/0000-0001-7260-4346)
- Ying Zhang (ORCID: https://orcid.org/0009-0003-5645-8299)
Institutions
- Institute of Special Animal and Plant Sciences (CN)
Publication Details
- Journal
- Cellular & Molecular Biology Letters
- Published
- 2025-11-13
- DOI
- https://doi.org/10.1186/s11658-025-00826-3
- Citations
- 2
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 3.76
Funders
- Natural Science Foundation of Jilin Province
- National Natural Science Foundation of China
- People's Government of Jilin Province
- Chinese Academy of Agricultural Sciences
- Agricultural Science and Technology Innovation Program