Shotgun metagenomic mining reveals a new FAD-dependent D-lactate dehydrogenase in an isopod gut microbiome
This study illustrates how metagenomics, structural biology, and computational tools can jointly drive the discovery of new enzymes with valuable biotechnological applications aligned with circular economic principles. The newly identified D-lactate dehydrogenase, PdG-D-LDH, exhibits thermostability, stereoselectivity, and high catalytic efficiency, providing new insights into the structure-function relationships of lactate-metabolizing enzymes.
Authors
- Patrícia T. Borges (ORCID: https://orcid.org/0000-0002-0602-819X)
- María Lorena (ORCID: https://orcid.org/0009-0004-4862-651X)
- Lı́gia O. Martins (ORCID: https://orcid.org/0000-0003-0082-9591)
- André Taborda (ORCID: https://orcid.org/0000-0002-3003-0580)
- Igor Tiago (ORCID: https://orcid.org/0000-0002-9301-3585)
- Catarina Coelho (ORCID: https://orcid.org/0000-0002-6760-367X)
- António Veríssimo (ORCID: https://orcid.org/0000-0003-4996-3185)
- Vânia Brissos (ORCID: https://orcid.org/0000-0002-8171-0113)
- Tomás Frazão (ORCID: https://orcid.org/0000-0001-5522-4085)
Institutions
- Hospitais da Universidade de Coimbra (PT)
- Instituto de Tecnología Química (ES)
- University of Coimbra (PT)
- Universidade Nova de Lisboa (PT)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2025-11-13
- DOI
- https://doi.org/10.1128/aem.01480-25
- Primary Topic
- Biochemical Acid Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00