Extraction, characterization, and efficacy enhancement of natural dye from locally grown plant impatiens tinctoria

In this Study, the phytochemical constituents and radical scavenging activity of the MeOH extract of Impatiens Tinctoria were investigated along with the dye capability of the crude tubers. Phytochemical screening has shown the presence of alkaloids, saponins, phenols, terpenoids, steroids, tannins, and glycosides in the MeOH extract of I. Tinctoria tubers. The MeOH extract of I. tinctoria tubers exhibited 39.07, 46.94, and 25.15% scavenging activity at the concentration of 1000 ppm using DPPH, ABTS, and H₂O₂ antioxidant activity assay, respectively. The methanol extract from the tubers showed an inhibition zone of 9.46 ± 0.03 mm in diameter for the gram-negative bacterium (E. coli). The dyeing of cotton fabrics using crude I. Tinctoria tubers were also evaluated. The effects of different mordants, AlK(SO₄)₂12H₂O, NaOH, HNO₃, CH₃COOH, NH_3, and lemon juice, were evaluated on the dyeing of cotton fabrics in crude I. Tinctoria. Treatment of cotton fabrics with NaOH resulted in a better appearance, with the most intense red colour. The study demonstrated that cotton fabrics with desired color depth and appearance could be prepared by varying the concentration of NaOH and immersion time in crude I. Tinctoria.