Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors
Overall, host engineering to adjust cellular conditions and ligand concentrations provides a versatile approach to enhance the sensitivity, precision, and efficiency of single-cell-based enzyme screening platforms.
Authors
- Han-Bae Kong (ORCID: https://orcid.org/0000-0003-2856-7060)
- Seung‐Goo Lee (ORCID: https://orcid.org/0000-0003-4539-9812)
- Haseong Kim (ORCID: https://orcid.org/0000-0002-4594-5791)
- Jinju Lee (ORCID: https://orcid.org/0000-0002-4141-2912)
- Bo-Young Yoo
- Jonghyeok Shin (ORCID: https://orcid.org/0000-0002-3520-8704)
- Eugene Rha
- Eun Jung Jeon (ORCID: https://orcid.org/0000-0002-9314-7997)
- Hyewon Lee (ORCID: https://orcid.org/0000-0003-2255-3418)
- Dae‐Hee Lee (ORCID: https://orcid.org/0000-0002-0423-9057)
- Kil Koang Kwon
Institutions
- Korea Advanced Institute of Science and Technology (KR)
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Sungkyunkwan University (KR)
- Korea University of Science and Technology (KR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-11-12
- DOI
- https://doi.org/10.1186/s13036-025-00574-0
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Korea Research Institute of Bioscience and Biotechnology
- Ministry of Science and ICT, South Korea