High-throughput excipient screening using 384-well plates and a pipetting robot: assessing protein stability after freeze-drying to pre-select viable formulations
There were no significant differences in enzymatic activity when comparing the automated 384-well plate freeze-drying method with a common in-vial method, while offering the added sustainability benefits of increased throughput, reduced workload, and lower protein and reagent usage. This approach might be suitable for the pre-selection of viable formulations.
Authors
- Henderik W. Frijlink (ORCID: https://orcid.org/0000-0001-7901-8198)
- Wouter L.J. Hinrichs (ORCID: https://orcid.org/0000-0002-1481-2769)
- Gerrit J. Poelarends (ORCID: https://orcid.org/0000-0002-6917-6368)
- Daan Zillen (ORCID: https://orcid.org/0000-0002-1033-3779)
- Ronald van Merkerk
- Noa N. van der Ploeg
Institutions
- University of Groningen (NL)
Publication Details
- Journal
- Drug Development and Industrial Pharmacy
- Published
- 2025-11-10
- DOI
- https://doi.org/10.1080/03639045.2025.2584353
- Primary Topic
- Protein purification and stability
- Type
- article
- Field-Weighted Citation Impact
- 0.00