Bis-Pseudoindoxyls: A Compact Fluorophore with Red-Shifted Absorption and Fluorescence for Bioimaging Applications
A new class of single benzene-based fluorophores, bis-pseudoindoxyls, was developed. The core scaffold <b>14CO25NH</b> is constructed via a visible-light-induced radical cyclization. Subsequent <i>N</i>-alkylation produced pronounced bathochromic shifts. The optimized dye <b>14CO25Nallyl</b> exhibited absorption and emission maxima at 621 and 687 nm, respectively, with a moderate quantum yield in CHCl<sub>3</sub>. These properties enable long-term live-cell imaging of lipid droplets in the red spectral region.
Authors
- Akiko Takaya (ORCID: https://orcid.org/0000-0003-1726-7150)
- Tetsuhiro Nemoto (ORCID: https://orcid.org/0000-0001-8858-161X)
- Masaya Nakajima (ORCID: https://orcid.org/0000-0002-5928-500X)
- Tomohiro Yazawa
Institutions
- Chiba University (JP)
- Chiba University Hospital (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-11-10
- DOI
- https://doi.org/10.1021/acs.orglett.5c04305
- Citations
- 1
- Primary Topic
- Photochromic and Fluorescence Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.52