Plant-Derived Extracellular Vesicles in Cosmetics: Building a Framework for Safety, Efficacy, and Quality

Plant-derived extracellular vesicles (PDEVs) are rapidly gaining popularity in cosmetics and regenerative medicine due to their biocompatibility, natural origin and promising bioactive properties. Nevertheless, the absence of standardized guidelines for their characterization has resulted in an inconsistent, unregulated landscape. This compromises product reproducibility, consumer safety, and scientific credibility. Here, a comprehensive set of minimal characterization guidelines for PDEVs is proposed to include physical and chemical profiling, molecular marker identification, cargo analysis, and stability assessment under storage and formulation conditions. Functional validation through cellular uptake assays, activity tests, and advanced in vitro or ex vivo models that replicate realistic skin exposure scenarios is pivotal. Requirements for transparent labelling, reproducible sourcing, batch-to-batch consistency, and biological activity substantiation to support claims related to skin regeneration, anti-aging, and microbiome modulation are also required. By establishing a harmonized baseline for quality and efficacy evaluation, these guidelines aim to elevate the scientific standards and promote the safe, ethical, and effective use of PDEV-based ingredients in cosmetic and biomedical applications.