Clinical Evaluation of a Mesotherapy Formulation for Weakened and Sparse Eyebrows With Assessment of Some Psychological and Social Aspects

ABSTRACT Background Eyebrows (EBs) are essential to facial symmetry, expression, and self‐image. EB thinning can negatively impact psychological and social well‐being. Mesotherapy (MT) has gained traction as a minimally invasive treatment for hair restoration, yet its impact on eyebrow regeneration and psychosocial outcomes remains under‐investigated. Aims This study assesses the psychological and clinical effects of eyebrow mesotherapy (EB MT) using a growth factor‐based serum in individuals with sparse or weakened eyebrows. Patients/Methods Thirty‐two participants (ages 18–65) underwent three sessions of EB MT at two—to 4‐week intervals. Treatment utilized “point by point” and “nappage” injection techniques with growth factor‐based serum (GF Advanced Hair Complex+, AQ Skin Solutions Inc., Irvine, CA, USA). This cosmetic‐grade formulation contains human fibroblast–conditioned media rich in growth factors and is manufactured under Good Manufacturing Practice (GMP) standards and FDA cosmetic compliance. The product was commercially purchased for clinical use, with no manufacturer involvement in study design or data interpretation.” Participants outcomes were evaluated using structured questionnaires measuring social engagement, quality of life, confidence, and mood. In addition, eyebrow width, length, and density were objectively measured across sessions. Statistical analysis was performed using Python (vX.X), applying non‐parametric tests. Results EB MT was associated with significant improvements participants' psychosocial scores, with mean values of 7.81 for social engagement, 6.71 for quality of life, 6.09 for confidence, and 6.13 for mood. Due to the ordinal nature of our outcome data (0–10 scales), we employed non‐parametric tests. A Spearman's rank correlation revealed a strong association between social engagement and quality of life ( ρ = 0.667, p < 0.001), a moderate association between confidence and mood ( ρ = 0.471, p < 0.01), and a weak, nonsignificant association between social engagement and confidence ( ρ = 0.152, p > 0.05). Furthermore, the Mann–Whitney U test confirmed that social engagement scores were significantly higher than confidence scores ( U = 916.0, p < 0.001). Objective measurements showed increased eyebrow width (+11 µm), length (+3.1 mm), and density (+8 hairs/cm²), all statistically significant ( p < 0.05). The treatment was well tolerated and showed consistent clinical improvement. Conclusions EB MT using growth factor‐based serum appears to be associated with clinical and psychological improvement in eyebrow restoration. It may enhance facial aesthetics and be associated with improved social engagement and emotional well‐being. These findings support broader use of EB MT in aesthetic dermatology.