ARROW: Allele-Specific Recombined gRNA Design for Reduced Off-Target with Enhanced Specificity
Compared with perfectly matched gRNAs, the strategic incorporation of mismatches into gRNAs enhanced editing specificity for single-base mutant alleles. Our findings substantially improve the precision and safety of CRISPR-based genome editing for cancer therapy, particularly in cases involving mutant alleles.
Authors
- Seunghun Kang (ORCID: https://orcid.org/0000-0001-6317-4321)
- Junho K. Hur (ORCID: https://orcid.org/0000-0003-3794-1149)
- Kyung Wook Been (ORCID: https://orcid.org/0000-0002-6946-2188)
- Woochang Hwang (ORCID: https://orcid.org/0000-0003-0489-3304)
- Sumin Hong (ORCID: https://orcid.org/0009-0004-8887-7874)
- Taegeun Bae
Institutions
- Hanyang University (KR)
Publication Details
- Journal
- Bioengineering
- Published
- 2025-11-12
- DOI
- https://doi.org/10.3390/bioengineering12111237
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Korea Basic Science Institute
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea