Evaluation of serological assays for intra vitam diagnosis of bovine tuberculosis in water buffalo (Bubalus bubalis)

The ELISA showed a specificity of 98.2%. However, sensitivity differed based on the antigen used: among the most reactive proteins, sensitivity was 67.5% for MPB70, 69.8% for P22, and 74.4% for PPDB. Moreover, approximately 70% of samples with discordant IDT and IGRA results, as well as those with positive IDT but inconclusive IGRA results, tested positive by serology, highlighting the potential of antibody-based detection to improve TB diagnosis in buffaloes. Our findings suggest that integrating serological testing with standard diagnostic methods could enhance the detection of infected animals, ultimately contributing to better TB control in buffalo populations.

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Publication Details

Journal
Frontiers in Microbiology
Published
2025-11-12
DOI
https://doi.org/10.3389/fmicb.2025.1684425
Citations
2
Primary Topic
Tuberculosis Research and Epidemiology
Type
article
Field-Weighted Citation Impact
2.79

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article

Evaluation of serological assays for intra vitam diagnosis of bovine tuberculosis in water buffalo (Bubalus bubalis)

Giovanni Parisio, Nicoletta Vitale, Mery Boifava, A. Viscito et al.
2 citations
Frontiers in Microbiology
Tuberculosis Research and Epidemiology
2.79
article

Evaluation of serological assays for intra vitam diagnosis of bovine tuberculosis in water buffalo (Bubalus bubalis)

Giovanni Parisio, Nicoletta Vitale, Mery Boifava, A. Viscito, Javier Bezos, María Beatrice Boniotti, Manuel J. Rodrı́guez, Alessandra Martucciello, Esterina De Carlo, Lorena Schiavo, Sara Giovannozzi, Inmaculada Moreno Iruela
article en
2 citations

Abstract

The ELISA showed a specificity of 98.2%. However, sensitivity differed based on the antigen used: among the most reactive proteins, sensitivity was 67.5% for MPB70, 69.8% for P22, and 74.4% for PPDB. Moreover, approximately 70% of samples with discordant IDT and IGRA results, as well as those with positive IDT but inconclusive IGRA results, tested positive by serology, highlighting the potential of antibody-based detection to improve TB diagnosis in buffaloes. Our findings suggest that integrating serological testing with standard diagnostic methods could enhance the detection of infected animals, ultimately contributing to better TB control in buffalo populations.

Frontiers in MicrobiologyVol. 16
Instituto de Salud Carlos III (ES), Centro Nacional de Microbiologia (ES), Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" (IT), Department of Animal Health (VN), Istituto Zooprofilattico Sperimentale del Mezzogiorno (IT), Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta (IT)
European Commission, Ministero della Salute
Openalex Percentile: Top 8%
Tuberculosis Research and Epidemiology
2.79
DOIOpen Access PDFOpenAlex
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