Fetal fractions mediate the association between total cell-free DNA and preeclampsia risk in a non-invasive prenatal testing cohort

This study observed that elevated total cfDNA levels during NIPT were associated with an increased risk of PE, with FFs acting as partial mediators. These findings warrant further investigation into total cfDNA and FFs as potential biomarkers for early PE risk stratification, even in high-risk settings such as twin pregnancies, to determine whether their use in screening could contribute to the development of strategies aimed at improving maternal-fetal health.