Design of antibody structure-guided epitope vaccines <i>in silico</i> to induce potent immune responses against emerging viruses

Public health emergencies pose significant threats to both human and environmental health, and the rapid control of emerging diseases often remains challenging due to their unknown characteristics. In this context, vaccines have historically been instrumental in the fight against major diseases, with epitope vaccines emerging as a preferred approach due to their precision and efficacy. In previous studies, we developed a strategy for peptide vaccine design based on simulated epitopes for tilapia lake virus (TiLV). To further improve vaccine immunogenicity and capitalize on the significance of antibodies for epitope screening, this study presents a structural modification strategy based on antigen-antibody docking for reverse vaccinology in conjunction with single amino acid mutagenesis inducing a robust immune response in tilapia. We believe that our findings are highly relevant to the field and contribute to the ongoing efforts to combat public health threats.