Biochemical and immunohistochemical evaluation of zinc oxide nanoparticles against experimental systemic amyloidosis in male rats

Forty male rats, two months old age were used in this experiment, divided into four groups, with ten animals per group. The first group was considered the control group. The second group was injected IP with nano zinc oxide at a dose of 5 mg/Kg B.wt. The third group was injected with 3 ml of 8% sodium caseinate the fourth group was treated with sodium caseinate and nano zinc oxide. The results of the histological examination of Sodium caseinate treated group kidneys showed coagulative necrosis in epithelial cells of the renal tubules with a Mesangial cells proliferation and deposition of amyloid in interstitial tissue and basement membrane of the epithelial cells and the wall of the blood vessels. The lesions were more severe after 60 days of treatment The GIV showed a clear improvement, especially after 60 days of treatment. In the spleen, the histological sections showed atrophy and deposition of amyloid in the red and white pulp in the GIII after 25 days of treatment, And the changes became more sever at the end of the experiment (60 days), which were represented by necrosis in the white and red pulp. The microscopic examination of the spleen samples of GIV showed a reduction in the pathological lesions. The results of histochemistry examination of kidney and spleen samples showed a positive reaction of amyloid deposition Immunohistochemistry examination, there was a strong reaction to the protein beta-amyloid in Kidneys and spleen in GIII a weak reaction in The GIV after 60 days of treatment.