Single-cell analysis of heterogeneity in reverted hiPSC-derived human hepatic stellate cells
Removing the cause of liver injury-curing hepatitis C or withdrawing lipotoxic stress-allows scar-forming liver cells (hepatic stellate cells) to partly revert to a healthier, vitamin-A-storing state; single-cell profiling reveals its heterogeneity and identify a subset nearing true quiescence. This rebound depends on intercellular interaction, in part on the immune signal IL-10 from macrophages, yet reverted cells remain easier to re-activate. These findings provide insights into dynamics of hepatic stellate cells and suggest potential therapeutic avenues for liver fibrosis by targeting stellate cell reversion.
Authors
- W Chen (ORCID: https://orcid.org/0009-0003-1677-0982)
- Zhiquan Feng (ORCID: https://orcid.org/0000-0002-7704-4197)
- Yifei Zheng (ORCID: https://orcid.org/0000-0001-9257-8464)
- Xianfang Wu (ORCID: https://orcid.org/0000-0002-2995-935X)
- Lu Bian (ORCID: https://orcid.org/0000-0001-7711-8929)
- Fan Zhang (ORCID: https://orcid.org/0000-0002-8813-2627)
- Lei Wang (ORCID: https://orcid.org/0000-0003-2402-2338)
- Xinjia Wang
- Eun Hee Ha (ORCID: https://orcid.org/0009-0007-7137-0331)
- Yujie Zhang (ORCID: https://orcid.org/0000-0002-5524-2790)
- Kyle O’Shaughnessy (ORCID: https://orcid.org/0009-0005-3517-7345)
- Andrea Hochwald (ORCID: https://orcid.org/0009-0002-9725-6717)
Institutions
- University Hospitals of Cleveland (US)
- Cleveland Clinic Lerner College of Medicine (US)
- Case Western Reserve University (US)
Publication Details
- Journal
- JHEP Reports
- Published
- 2025-11-11
- DOI
- https://doi.org/10.1016/j.jhepr.2025.101669
- Primary Topic
- Liver physiology and pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Cleveland Clinic
- National Institutes of Health